Messenger Characteristics of Nascent Bacteriophage RNA

Messenger characteristics of nascent bacteriophage RNA.

The proteins initiated in vitro by nascent bacteriophage f2 RNA strands attached to isolated replicating structures have been analyzed. The observations confirm that coat protein is the major product initiated and completed. Nascent strands direct the initiation of viral maturation protein in amounts similar to the maximum levels observed in vivo; this synthesis is independent of translation of...

Messenger RNA.

The rZZA Gene of Bacteriophage T 4 . 11 . Regulation of Its Messenger RNA Synthesis

When the rZZ genes are first introduced into cells which had been previously infected by T4 phage deleted for these genes, the kinetics of synthesis of rZZA and rZZB RNA are rapid and identical. We show that this rapid synthesis depends on a functional motA gene for rZZB, but not for rZZA, RNA synthesis. By primer-extension mapping of T4 messenger RNA, we find three promoters close to the rZZA ...

Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide

The cytoplasmic fates of mRNAs are influenced by interactions between RNA-binding proteins and cis regulatory motifs. In the cytoplasm, mRNAs are present as messenger ribonucleoprotein particles, which include not only proteins that bind directly to the mRNA, but also additional proteins that are recruited via protein-protein interactions. Many labs have sought to purify such particles from cel...

Who discovered messenger RNA?

The announcement of the discovery of messenger RNA (mRNA) and the cracking of the genetic code took place within weeks of each other in a climax of scientific excitement during the summer of 1961. Although mRNA is of decisive importance to our understanding of gene function, no Nobel Prize was awarded for its discovery. The large number of people involved, the complex nature of the results, and...